rabbit anti syn2  (Proteintech)

Journal: Frontiers in Immunology

Article Title: Maternal synapsin autoantibodies are associated with neurodevelopmental delay

doi: 10.3389/fimmu.2023.1101087

Figure Lengend Snippet: Phenotype of children of mothers tested for SYN1 autoantibodies. Developmental delay in the domains speech and motor skills were the most common findings, often associated with intellectual disability and epilepsy. ID, intellectual disability; ASD, autism-spectrum disorder.

Article Snippet: IgG AF488-antibody (Dianova, #109-545-003), commercial rabbit SYN1/SYN2 antibody (Synaptic Systems, #106002) and anti-rabbit IgG AF594-antibody (Jackson IR, #111-585-003) were used to detect IgG binding.

Techniques:

Journal: Frontiers in Immunology

Article Title: Maternal synapsin autoantibodies are associated with neurodevelopmental delay

doi: 10.3389/fimmu.2023.1101087

Figure Lengend Snippet: Detection of synapsin 1 (SYN1) autoantibodies. (A) Examples of immunofluorescence stainings, using human sera at a 1:300 dilution (green) with or without binding to HEK cells overexpressing human SYN1b are shown in the top row and bottom row. Using a commercial SYN1/2 antibody, protein expression is verified (red). DAPI is used to stain the nuclei (blue). Scale bar: 20 µm. (B) In 9.6% of mothers SYN1 autoantibodies were detected using a CBA. (C) CBA and in-house ELISA correlated weakly, with higher mean serum IgG levels in the CBA-positive group. (D) Representative immunoblots of wild type (WT) and Syn1/2/3 triple KO (TKO) mice cortex homogenates with a commercial synapsin 1/2 antibody as positive control, and with sera (1:200 dilution) from a CBA-positive mother and a CBA-negative control. Major bands at 80-90 kDa corresponding to the molecular weight of synapsin 1a/1b and additional bands at 50-55 kDa that could represent the synapsin 2b isoform or breakdown products of synapsin 1 were detected in wild type but not in TKO mouse tissue by both the commercial antibody and the serum of the CBA-positive mother. Detection of GAPDH served as loading control. HEK human embryonic kidney; CBA, cell-based assay; ELISA, enzyme-linked immunosorbent assay, WB, western blot.

Article Snippet: IgG AF488-antibody (Dianova, #109-545-003), commercial rabbit SYN1/SYN2 antibody (Synaptic Systems, #106002) and anti-rabbit IgG AF594-antibody (Jackson IR, #111-585-003) were used to detect IgG binding.

Techniques: Immunofluorescence, Binding Assay, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Positive Control, Negative Control, Molecular Weight, Cell Based Assay

Journal: Frontiers in Immunology

Article Title: Maternal synapsin autoantibodies are associated with neurodevelopmental delay

doi: 10.3389/fimmu.2023.1101087

Figure Lengend Snippet: Phenotype of patients with positive maternal synapsin I (SYN1) autoantibodies.

Article Snippet: IgG AF488-antibody (Dianova, #109-545-003), commercial rabbit SYN1/SYN2 antibody (Synaptic Systems, #106002) and anti-rabbit IgG AF594-antibody (Jackson IR, #111-585-003) were used to detect IgG binding.

Techniques:

Journal: Frontiers in Immunology

Article Title: Maternal synapsin autoantibodies are associated with neurodevelopmental delay

doi: 10.3389/fimmu.2023.1101087

Figure Lengend Snippet: Clinical phenotype in patients of mothers with autoantibodies against synapsin 1 (SYN1). (A, B) Forest plot and bar chart showing the significantly increased risk for intellectual disability and behavioral problems. ID, intellectual disability; ASD, autism-spectrum disorder. * p ≤ 0.05.

Article Snippet: IgG AF488-antibody (Dianova, #109-545-003), commercial rabbit SYN1/SYN2 antibody (Synaptic Systems, #106002) and anti-rabbit IgG AF594-antibody (Jackson IR, #111-585-003) were used to detect IgG binding.

Techniques: